Figure 2.

ASRs encode SG6 R2R3‐MYB proteins. (a) The ASR gene cluster is present in P. inflata, P. violacea, and in the P. hybrida lines M1 and V30 but is absent in P. axallaris, P. parodii, P. exserta, and in the P. hybrida lines W115, R27. A PCR method was used to amplify the specific ASR genes with their specific primers from DNA. (b) ASR1 and ASR2 encode truncated proteins in P. inflata. The mutations are indicated by an asterisk in the sequences. (c) Phylogenetic analysis of SG6 proteins in different species. The tree was constructed with maximum likelihood based on multiple alignments of the amino acid sequence of the R2R3‐motif only. (d) ASR genes from P. inflata and the P. hybrida line M1. Exons in ASR genes are indicated as solid boxes; similar promoter regions are shown as solid arrows of the same color, open triangles indicate insertions. Drawings are not to scale. Red crosses indicate mutation interrupting coding sequences