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. 2019 Jan 21;3(1):e00114. doi: 10.1002/pld3.114

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© 2019 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Overexpression of ASRs ^M1 enhance anthocyanin levels in the an2 an4 double mutant (Petunia hybrida line W115). (a) Flowers at development stages of 2, 4, and 7, and vegetative plant parts. Red size bars equal 5 mm. (b) Anthocyanin content in young leaves of untransformed control W115 and ASRs ^M1‐OE plants indicated as the mean ± SD of three biological replicates. (c) Analysis of the expression of F3′5′H and DFR genes in young leaves of W115 untransformed control and ASRs ^M1‐OE plant. Relative expression is indicated as the mean ± SD of three biological replicates. Statistical significance was determined by one‐way ANOVA and significant differences between means is indicated by an asterisk (p‐value < 0.05)