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. 2018 Aug 10;2(8):e00081. doi: 10.1002/pld3.81

Figure 4.

Figure 4

Disruption of the NLS in SlySBP8b and SlySBP12a prevents cell death in N. benthamiana upon overexpression. 35S:HA‐SlySBP8b, 35S:HA‐SlySBP8b(NLS mt ), 35S:HA‐SlySBP12a, or 35S:HA‐SlySBP12a(NLS mt ) were transiently transformed in N. benthamiana. (a) Images of leaves taken 5 days post‐transformation. (b) Electrolyte leakage assay used to quantify cell death. 35S:YFP—blue diamond; 35S:HA‐SlySBP8b—red square; 35S:HA‐SlySBP8b(NLSmt)—green triangle. Two independent experiments were pooled together for a total of 13 biological replicates for each gene. Error bars represent a 95% confidence interval. (c) Electrolyte leakage assay used to quantify cell death. 35S:YFP—blue diamond; 35S:HA‐SlySBP12a —red square; 35S:HA‐SlySBP12a(NLSmt)—green triangle. Three independent experiments were pooled together for a total of 22 biological replicates for each gene. Error bars represent a 95% confidence interval. (d) Immunoblot performed on tissue collected 2 days post‐transformation. An α‐HA antibody was used to detect SlySBP proteins, and Ponceau S stain was used to detect Rubisco as a loading control