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. 2018 Aug 10;2(8):e00081. doi: 10.1002/pld3.81

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© 2018 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Removal of the TMD from SlySBP12a results in enhanced cell death upon overexpression. 35S:HA‐SlySBP12a, 35S:HA‐SlySBP12a(ΔTMD), or 35S:YFP was transiently transformed in N. benthamiana. (a) Images of leaves taken 5 days post‐transformation. (b) Electrolyte leakage assay used to quantify cell death. 35S:YFP—blue diamond; 35S:HA‐SlySBP12a —red square; and 35S:HA‐SlySBP12a(ΔTMD)—green triangle. Three independent experiments with similar results were pooled together for a total of 22 biological replicates for each gene. Error bars represent a 95% confidence interval