Figure 8.

Endoplasmic reticulum localization of SlySBP12a and TMD_{S ly SBP 12a} in tomato protoplasts. Tomato protoplasts were transfected with plasmids encoding 35S:YFP‐SlySBP12a or 35S:YFP‐ TMD_{S ly SBP 12a} and imaged by CLSM. An SP‐mCherry‐HDEL construct was cotransfected to serve as an ER marker (red). The magenta signal represents chloroplast autofluorescence. (a) Representative images of tomato protoplasts expressing 35S:YFP‐SlySBP12a or 35S: TMD_{S ly SBP 12a} with the ER marker. Numbered regions indicated by arrows were used for colocalization analysis. (b) Intensity histograms of the four regions selected for colocalization analysis. Pearson's R values and Costes p‐values are displayed for each region