Figure 10.

Overexpression of SlySBP8b and SlySBP12a enhances A. alternata growth on N. glutinosa. 35S:YFP‐SlySBP8b, 35S:YFP‐SlySBP12a, or 35S:YFP was transiently transformed in N. glutinosa. As a positive control for cell death induction, leaves were treated with 5 μM FB1. Agar plugs containing actively growing A. alternata mycelium were placed fungal‐side‐down on leaves. Images were taken, and lesion areas were recorded, 3 days after fungal inoculation. (a) Quantification of lesion area using ImageJ. The results of four randomized and blind experiments were pooled representing 54 leaves for each treatment. Data are displayed as a dotplot with the medians represented by red horizontal lines. Treatments with the same letter are not statistically significant as determined by a one‐way ANOVA with Tukey's HSD post hoc test (YFP/SBP8b, p = 0.02; YFP/SBP12a, p = 2.0E‐7; YFP/FB1, p = 5.0 E‐7; SBP8b/SBP12a, p = 0.02; SBP8b/FB1, p = 0.04). (b) Images of inoculated leaves with lesions outlined by a dotted white line