Figure 5.

The growth and developmental phenotypes of MATE45 mutants are abscisic acid (ABA)‐dependent. (a) Germination of seeds on agar‐based media. Seeds were imbibed for 3 days at 4°C, germination rates following the imbibition treatment are shown. (b) Percent seed germination after 48 hr on agar media containing different concentrations of ABA. Germination was scored by the visible protrusion of the radical from the seed coat. The average from three plates of 50 seeds is shown. (c) Amount of ABA from seeds imbibed at 4°C for 24 hr measured by LC‐MS ⁿ. Error bars represent the standard error of the mean (n = 4 biological replicates). ^aLess than control, p < 0.05; two‐tailed Student's t test. (d) qRT‐PCR measurements of gene expressions relative to ACTIN2. ^aLess than control, ^bgreater than control, p < 0.05; two‐tailed Student's t test. (e) Staining of ABA signaling marker pRD29B:GUS in germinating seeds. Cotyledons of mate45‐1 generally stained more intensely than cotyledons of the wild‐type. Scale 500 μm. (f) Seeds in (e) with seed coat removed. Scale 250 μm