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. 2018 Oct 12;2(10):e00087. doi: 10.1002/pld3.87

Figure 6.

Figure 6

Accelerated true leaf primordia initiation of mate45‐1 in anthocyanin induction condition (AIC) is abscisic acid (ABA)‐dependent. (a) True leaf primordia growth phenotype of lines identified by our screen. mate45‐1 and abcg25‐5 seedlings generally had visible true leaf primordia at 10 dag in AIC. Scale 250 μm. Percentage of seedlings that had visible true leaf primordia at 12 dag in AIC: (b) Mutants identified by our screen (n = 4 biological replicates). (c) ABA‐deficient mutants aba2‐1 and aba3‐1, and the mate45‐1 aba2‐1 double mutant. mate45‐1 aba2‐1 restored wild‐type rates of primordia initiation (n = 4 biological replicates). (d) Seedlings treated with 10 μM ABA biosynthesis inhibitor fluridone (Flur), 3 μM ABA, or solvent (DMSO) (n = 4 biological replicates). (e) ABA amounts from lyophilized seedlings at 5 dag in AIC. Two‐way ANOVA, Tukey post hoc test (p < 0.01); different letters show significant differences (n = 4 biological replicates)