Figure 3.

Validation of RNA‐seq data. qRT PCR was used to validate the expression patterns of select differentially expressed genes in male nectaries identified through RNA‐seq analyses. The fold‐change in each stage was determined relative to 0 hr (the secretory stage [anthesis] was set as 1). Statistically significant differences in expression between the stages are noted by different letters (pairwise, two‐tailed t test, p < 0.05). The colored boxes represent the corresponding fold‐change observed by RNA‐seq analysis. SBE2 = Starch Branching Enzyme 2, CWINV4 = Cell Wall Invertase 4, AMY3 = Alpha‐amylase 3, BAM1 = Beta‐amylase 1, SUS5 = Sucrose Synthase 5. MYB305 and SWEET9 are the full gene names