Figure 3.

cesa4ko is rescued only by the CESA744 chimeric construct. Of 12 chimeric CESAs tested, only CESA744 provided phenotypic rescue of cesa4ko (irx5‐4) along with the two positive controls of CESA4 driven by native promoter fragments of CESA4 or CESA7 (Pro4:CESA4 and Pro7:CESA4). (a) Cellulose content of lines (see Figure 2 for details). (b) Stem height measurements for each line (n = 7–26 stems with STD error bars). In (a) and (b), asterisks indicate partially rescued lines (p < 0.01 as determined by nonparametric Mann–Whitney test between values for the wild type and the transformed line). (c) Immunoblot analysis showed that, as expected, no signal was observed in the rescued CESA744 line with anti‐CESA4.1, whereas a doublet was detected when probing with anti‐CESA7.1. Anti‐CESA7.3 did not recognize the 5 kDa larger CESA744, but endogenous CESA7 was recognized. Anti‐CESA8.2 confirmed the presence of CESA8