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. 2018 Jul 10;2(7):e00061. doi: 10.1002/pld3.61

Figure 4.

Figure 4

cesa7ko is rescued by the CESA747 chimeric construct. Of 12 chimeric CESAs, only CESA747 rescued the cesa7ko (irx3‐4) mutant phenotype, along with the positive control of Pro7:CESA7. CESA4 driven by the native CESA7 promoter (Pro4:CESA4) did not rescue. (a) Cellulose content of lines (see Figure 2 for details). (b) Stem height measurements for each line (n = 6–26 stems with STD error bars). In (a) and (b), asterisks indicate partially rescued lines (p < 0.01 as determined by nonparametric Mann–Whitney test between values for the wild type and the transformed line). (c) Immunoblot analysis showed normal CESA4 and CESA8. Furthermore, as expected, no signal was observed in the rescued CESA747 line using anti‐CESA7.3 that recognizes the central domain, while a 5 kDa larger protein is observed when probing with anti‐CESA7.1