Figure 5.

Truncation of the CESA8 N‐terminus does not abolish function. A truncated version of CESA8 (CESA8^ΔNT) lacking 153 amino acids, or 84% of the 182 amino acids in the N‐terminus, rescued the phenotypes of cesa8ko. (a) Cellulose content of wild type, cesa8ko, and two independent CESA8^ΔNT transgenic lines (see Figure 2 for details). (b) Stem height measurements for each line (n = 24–39 stems with STD error bars). In (a) and (b), asterisks indicate partially rescued lines (p < 0.01 as determined by nonparametric Mann–Whitney test between values for the wild type and the transformed line). (c) Images of plants showing good phenotypic rescue with CESA8^ΔNT in comparison with the wild type and the cesa8ko. (d) Immunoblot analysis of CESA8^ΔNT showed normal CESA4 and CESA7 and that the lower MW truncated form of CESA8 can be visualized when probing with anti‐CESA8.2