Figure 1.

Analysis of the three classes of transformants: control (Cambia 2300), 35S‐SPS, and 35S‐GS plants for the integration and functionality of the gene constructs. (a) DNA isolated from three independent transformants for each class was isolated and subjected to genomic PCR using a NPTII specific primer set. The products were then fractionated on agarose gels. (b) RNA isolated from the leaves of the same plants used in panel A were subjected to RT‐PCR using primer sets specific for the SPS transgene, the GS transgene, and Actin and the products were electrophoresed. (c) RNA isolated from the nodules of the same set of plants used in panels A and B were subjected to RT‐PCR using primer sets specific for SPS transgene, GS transgene, and Actin and the products were subjected to electrophoresis