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. 2019 Jan 31;3(1):e00115. doi: 10.1002/pld3.115

Figure 7.

Figure 7

Analysis of transcript accumulation for the endogenous SPS genes in the nodules of Control, 35S‐SPS, and 35S‐GS transformants. (a) A quantity of 2 μg of total RNA isolated from the nodules of the same set of plants used for protein analysis was subjected to semiquantitive RT‐PCR using primer sets specific for GS1a,GS1b,SPSA,SPSB, and Actin (internal control for RNA concentration). Following amplification, the products were subjected to electrophoresis. Data from a representative experiment are shown here. (b) The band intensities were measured using the KODAK image software. (c) The ratio of the band intensity obtained for GS1a,GS1b,SPSA,SPSB genes relative to the band intensity obtained with the Actin primer set was calculated for each transformant. The values obtained for the three independent transformants representing the 35S‐SPS and 35S‐GS classes were then compared with the average value obtained for the three control plants and plotted as bar graphs. Significant differences from the control plants were evaluated by ANOVA contrast test and shown by asterisks (*p < 0.05 or **<0.01)