Table 1.

Primers used to monitor the expression of the GS and SPS genes in the alfalfa transformants

Gene	Primer direction	Sequence
Medicago sativa Actin	Forward	5′‐ACTTAACCCAAAGGCCAATAGA‐3′
	Reverse	5′‐TGCTCATACGGTCAGCAATAC‐3′
M. sativa SPSA	Forward	5′‐CCACTCACTTGGTCGAGATAAG‐3′
	Reverse	5′‐TATGCCACTTGCCCATACAG‐3′
M. sativa SPSB5	Forward	5′‐CTCTTCACGCGCCAAATATCATCTC‐3′
	Reverse	5′‐AGCTCTTCCGCTTCTATCCTTCTC‐3′
M. sativa GS1a	Forward	5′‐CACAAATCAAGCTCCAGGACAAGATAG‐3′
	Reverse	5′‐CATCTCCAGCAGAAATACCAACAGAAG‐3′
M. sativa GS1b	Forward	5′‐TGGTCCCTCAGTTGGTATCT‐3′
	Reverse	5′‐TGTGTGTGTGGTGGCTTATG‐3′
Zea mays SPS1	Forward	5′‐CCCGAAGAAGAACATCACTACC‐3′
	Reverse	5′‐CGTCGATGTCATCTCTGTTACC‐3′