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. 2018 Nov 12;2(11):e00085. doi: 10.1002/pld3.85

Figure 3.

Figure 3

Positional cloning of SbNAC_D. (a) The marker 6_50878497_SNP had the highest LOD score in the QTL interval and was used to define a 2‐LOD interval spanning the QTL (purple). (b) RILs from BTx623*IS3620c (RIL 470, RIL 390, RIL 75, RIL 272) with breakpoints within the 2‐LOD interval were identified and phenotyped for the AER formation at anthesis. AER ratings are indicated in parentheses next to the RIL label to the left of the diagram. Blue haplotypes correspond to IS3620c alleles (+AER), and red haplotypes correspond to BTx623 alleles (−AER). The fine‐mapped interval contained eight genes. (c) Sobic.006G147400 (SbNAC_D) was identified as the best candidate for the “D” gene. The BTx623 allele contains a T‐to‐C polymorphism that causes a Gln to STOP (Q39X) change in the first exon of BTx623 (red line)