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. Author manuscript; available in PMC: 2019 May 9.
Published in final edited form as: Cell Rep. 2019 Mar 26;26(13):3574–3585.e3. doi: 10.1016/j.celrep.2019.02.109

Figure 1. In Situ Dual RNA Sequencing of Host-Pathogen Transcriptomes.

Figure 1.

(A) Experimental design for dual RNA-seq RNA extraction, library preparation, and sequence processing.

(B) Unsupervised clustering analysis of both M. leprae and human gene expression across leprosy skin lesions (coefficient of variation, >1.0). Heatmap represents expression of genes (rows) and patients (columns) in which red indicates a higher and green a lower expression Z score per gene across patients (n = 24).

(C) Ratio of the sum of all M. leprae gene abundances over the total sum of human or M. leprae gene abundance across L-lep patient lesions.

(D) Correlation plots of M. leprae abundance (log2 scale) against indicated Signature Visualization Tool (SaVanT) signature values (Z score) for IFN-b, IFN-g, CD4+ T cells, or plasma cells per L-lep patient. p value by Student’s t test (n = 9).

See also Table S1 and Figures S1 and S4.