Figure 5.

Ncad/GFP transport is dependent on cell-cell contacts. (A–C) Images of Ncad/GFP-expressing cells were captured every 5 s for 5 min. Projections of all video frames in a movie sequence are shown with the contrast inverted to better visualize the transport paths. Distinct trafficking behaviors are observed in isolated (A), recently (B) or fully contacting cells (C). Circles indicate vesicles with random Brownian-like motion. Inset in B illustrates two centrifugal (1 and 2) and one centripetal (3) pathway. Bar, 10 μM.