Figure 7.

Ncad/GFP-containing vesicles cofractionate with microsomes and MTs. (A) Schematic protocol of the different purification steps. (B) SDS-PAGE and immunoblot analysis of Ncad/GFP in the microsomal fraction (C2), MAP-depleted supernatant (S3), and MT/MAP fraction (C3) prepared from isolated, contacting, or Nz-treated isolated Ncad/GFP-expressing cells. Immunoblot detection of a known MAP protein, TOGp, is shown as a control. The amount of tubulin in the fractions is monitored by ponceau red staining of the membranes (middle panels) and immunoblot analysis by using an anti-α-tubulin antibody (bottom panels).