Table 1.

Distinct behavior for the traffic of the Ncad/GFP-containing vesicles in isolated or early contacting cells This table displays data summarizing frequency of centrifugal versus centripetal motion events of Ncad/GFP-containing vesicles. Results are means (±SEM) of several experimental performed in either isolated or early contacting Ncad/GFP-expressing cells (r = 9–10 cells). MetaMorph software has been used to measure the movement of the Ncad/GFP structures (tubules and individual vesicles) between frames from a continuously acquired live image (stack). We have tracked the positions with respect to a defined origin. Data regarding the X and Y coordinates and displacement of the vesicles were logged to disk and treated with Excel software.

Features of Ncad/GFP vesicles in	Isolated cells	Early contacting cells
% of centripetal flow	29	12
% of centrifugal flow	47	79
% stop phases	23	8.7
Velocities (μm/s)	0.18  ± 0.06	0.35  ± 0.1