Figure 1. Anti-PD-L1 antibody (Ab) with a CBP/β-catenin inhibitor decreases metastatic tumor growth in the liver.
Male C57BL/6J mice were intrasplenically injected with SL4 cells (5 × 105 cells) and treated with or without anti-PD-L1 Ab and/or PRI-724. The animals were humanely sacrificed 14 days post inoculation (A, B, D). (A) The livers were excised and photographed (left panels). Liver weights were measured (right panel). (B) Expression of Ki67 in the metastatic liver tumor (loupe magnification) was examined by immunohistochemistry using an anti-Ki67 Ab. Intrahepatic tumor load is presented as Ki67-positive areas based on the measurement of two non-sequential for each animal (graph on right panel). The pictures shown are representative of at least four independent experiments. Results are provided as box-and-whisker plot or as means ± SD of data collected from at least four independent experiments. *P < 0.05 based on the Kruskal-Wallis test (A) and one-way ANOVA test (B). (C) The survival rates of the animals are shown. Statistically significant differences were determined by performing a log-rank test. (D) Serum ALT levels were determined and the results are provided as means ± SD. n. s.; not significant.