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. 2019 Apr 30;10(32):3013–3026. doi: 10.18632/oncotarget.26892

Figure 4. Role of T cells in the anti-tumor effect of anti-PD-L1 antibody (Ab) and/or PRI-724.

Figure 4

Male C57BL/6J mice were intrasplenically injected with SL4 cells (5 × 105 cells). The animals were treated with or without anti-PD-L1 Ab and PRI-724 and were humanely sacrificed 5 days after inoculation. (A) Intrahepatic leukocytes (IHLs) isolated from mice inoculated with SL4 cells were stained for PD-1, CD44, and CD62L in CD8 and CD4 T-cells. The numbers represent the percentage of PD-1-, CD44-, and CD62L-positive and negative cells among the CD8 and CD4 T-cells. (B) Results are representative of three independent experiments. Significant relationships are indicated by P-values based on a two-tailed Student’s t-test. *P < 0.05. (C) The numbers represent the percentage of IFN-γ-, CD107a-, and IL-10-positive and negative cells among CD8 and CD4 T-cells. (D) Results are representative of three independent experiments. Significant relationships are indicated by P-values based on a two-tailed Student’s t-test. *P < 0.05.