Figure 3. Hydrogen peroxide adaptation involves female-specific increases in proteasomal proteolytic capacity.

(A-F) Male and female progeny were collected and placed onto 5% sucrose for 24 hours prior to H₂O₂ pretreatment [0μM-1000μM] for 8 hours, at which point, flies were transferred to 5% sucrose for a 16-hour recovery before collection. Proteolytic capacity was assessed in whole fly lysate by degradation of the fluorogenic peptide, Suc-LLVY-AMC. (A,C,E) In all three strains, males show no change in proteolytic capacity, irrespective pretreatment. (B,D,F) Females from all three strains show higher levels of proteolysis upon H₂O₂ pretreatment. Error bars denote the standard error of the mean (S.E.M) values. * P <0.05, ** P < 0.01, and *** P < 0.001, relative to control using one-way ANOVA.