Figure 4.

rBTNL2-Ig increases the generation of Tregs and decreases the activation of T cells in the spleen. The spleens were harvested from rBTNL2-Ig or control Ig-treated NOD mice at week 32 of age. The splenocytes were analyzed for the percentages of (A, B) CD4⁺ CD25⁺Foxp3⁺ Tregs, (C, D) CD69⁺ cells in CD4 and CD8 T cells, (E, F) IFNγ-producing CD4 and CD8 T cells, and (G-J) CD44⁻CD62L⁺ naïve, CD44⁺CD62L⁻ effective memory, and CD44⁺CD62L⁺ central memory CD4 and CD8 T cells. (A, C, E, G, I) Representative flow cytometric profiles and (B, D, F, H, J) statistical analysis of means ± SEM. *P < 0.05, ** P < 0.01 vs control Ig. The data are representative of 3 independent experiments with 4–6 mice per group.