Figure 5.

rBTNL2-Ig treatment attenuates insulitis in NOD mice. Pancreata were harvested from control Ig- and rBTNL2-Ig-treated NOD mice at 32 weeks of age. Sections of pancreata were subjected to H&E staining, or immunofluorescent staining with anti-insulin and anti-CD45 antibodies. (A) Representative sections of H&E and immunofluorescent staining. (B) Insulitis scores based on H&E staining. Scores were given as follows: score 1, free of infiltrates; score 2, <25%; score 3, 25–50%, and score 4, >50% infiltrates per islet. (C) Percentages of inflammation and loss of islets calculated according to the severity of intra islet infiltrates and insulin staining, respectively. The data are representative of 3 independent experiments with 4–6 mice per group.