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. Author manuscript; available in PMC: 2019 May 9.
Published in final edited form as: Methods Mol Biol. 2017;1634:33–53. doi: 10.1007/978-1-4939-7144-2_3

Fig. 2.

Fig. 2

Vacuum-enabled reagent introduction setup. The vacuum line is directly connected to a precision vacuum regulator and pressure gauge, which are then connected to a filter flask that is attached to tubing with a truncated pipette tip at the end. The pipette tip is pressed up against the microfluidic device inlet/outlet to withdraw the reagent solution into the device