Figure 5. SRT1720 activated SIRT1 to rescue oxidative stress-induced rat NP cells senescence.

The rat NP cells were divided into control, H₂O₂ treatment group and SRT1720 pretreatment group. The expression of SIRT1 and some senescence relative proteins (p53, p21, p16 and p-Rb) were detected using Western blot showed in (A) and (B). β-actin was used as an internal control. (^*P<0.05, ^**P<0.01, ^***P<0.001 vs control group) (C) Pro-inflammatory factors (TNF-α, IL-1β, IL-6 and IL-8) were detected by RT-PCR. (^*P<0.05, ^**P<0.01, ^***P<0.001 vs control group) (D) and (E) The cell cycle was detected by flow cytometry. (^*P<0.05, ^**p<0.01, ^***P<0.001 vs control group) (F) β-galactosidase staining was used to detect senescent rat NP cells. Scale bars 100 μm.