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. 2019 May 9;9:7129. doi: 10.1038/s41598-019-43584-x

Table 3.

Gene-specific primers used for qPCR in adrenal and pituitary tissue.

Gene Accession Orientation Primer Sequence (5′ - 3′) Product Length (bp)
LBR NM_205342 Forward GGTGTGGGTTCCATTTGTCTACA 80
Reverse CTGCAACCGGCCAAGAAA
MC2R NM_001031515 Forward ATTGCCTCACTGCCAACCAG 160
Reverse AGCAGGCACAGTAAGGGTTG
StAR NM_204686.2 Forward AGTGATGGCCCTTATCTCGGT 103
Reverse GGTGGCTGCTACAAACACTGC
HSD3B2 NM_205118.1 Forward GCCAAAGAGGAGCAAACCAG 203
Reverse TTCACCTCAGTCTTGCCCTG
POMC NM_001031098 Forward ATTTTACGCTTCCATTTCGC 141
Reverse AATGGCTCATCACGTACTTGC
CRHR1 NM_204321 Forward CCCTGCCCCGAGTATTTCTA 141
Reverse CTTGCTCCTCTTCTCCTCACTG
VT4R NM_001110438 Forward GGTTGCAGTGTTTTCAGAGTCG 137
Reverse CAAGATCCGCACCGTCAAG