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. 2019 May 9;9:7154. doi: 10.1038/s41598-019-43613-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VITVO technology and potentials. (a) Picture of the VITVO platform. (b) VITVO cartoon section showing structure and parts. (c) VITVO loading of cell suspension with a syringe allows colonization of the 3D matrix inner core. (d) Cells can be directly visualized in VITVO under a fluorescence microscope; cellular viability and growth can be monitored using luminescence or fluorescence with a plate reader instrument; VITVO system is closed, thus the device is easily transported and can be shipped; methacrylate embedding of 3D tissue rebuilt in VITVO allows histological studies on tissue slices.