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. 2019 May 9;9:7154. doi: 10.1038/s41598-019-43613-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In VITVO evaluation of sTRAIL pro-apoptotic action on Ewing sarcoma using two different luminometric approaches. (a) In VITVO dsRED⁺ A673 visualization using fluorescence microscopy at seeding and after 72 hours. Scale bar 100 μm. (b) In VITVO monitoring of sTRAIL action against tumor using microscope visualization of fluorescent cells during treatment (0 hour and 24 hours). (c) In VITVO measurement of dsRED⁺ A673 viability based on RealTime-Glo. Cell growth was monitored for 72 hours (left panel), then sTRAIL was added in VITVO and cell viability was measured after 24 hours based on luminescence (right panel). Untreated dsRED⁺ A673 cells were used as control. All measurements were performed in triplicate and data are expressed as means ± SD *P = 0.003. (d) In VITVO measurement of Luc⁺ A673 viability by the addition of luciferin. Cell growth was monitored for 72 hours (left panel), then sTRAIL was added in VITVO and cell viability was measured after 24 hours based on luminescence (right panel). Untreated Luc⁺ A673 cells were used as control. All measurements were performed in triplicate and data are expressed as means ± SD, *P = 0.002.