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. 2019 May 3;10:410. doi: 10.3389/fgene.2019.00410

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Copyright © 2019 Gao, Xu, Chen, Xue, Pan, Zhou, Ma and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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his-41p::his-41::mCherry transgene expression. (A) Representative fluorescence pictures of HIS-41::mCherry fusion protein expression in an smn-1(ok355)/hT2 heterozygous or an smn-1(ok355) homozygous animal. (B) RT-qPCR quantification of his-41::mCherry expression levels. (C) Quantification of the total fluorescence intensity (left) and density (right) of mCherry. For mRNA expression, each dataset is the average of three biological replicates from one transgenic line. For mCherry intensity, each dataset is the average of 20 transgenic animals in two transgenic lines, with 10 animals from each line. Statistics: two-tailed unpaired Student’s t-test. ^∗p < 0.05. Error bars: standard errors.