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Quantified sialic acid (indicated in micrograms per milliliter) of highly pure NTHi LOS grown on RPMI media supplemented with various molarity ratios of Neu5Ac and Neu5Gc. At a 1:1 ratio, the amount of Neu5Ac measured was found to be significantly (4-fold) greater than that of Neu5Gc (n = 3, P = 0.0210). The P value was calculated using a two-tailed unpaired Student's t test.
