FIG 8.

3′-Sialyllactose and 6′-sialyllactose both inhibit LPS-induced inflammatory responses via the NMHC-IIA–DAP12-Syk pathway in PAMs. (A to D) PAMs were transfected with indicated siRNAs targeting the NMHC-IIA–DAP12-Syk pathway and stimulated with LPS (10 μg/ml) for different time periods (0, 15, and 30 min) in the presence of 3′-sialyllactose or 6′-sialyllactose (10 μM). IB was performed to detect p65 phosphorylation. Experiments in all panels were repeated at least three times, and similar results were obtained. Quantitation data are mean ± SD (n = 3). Statistical analysis used in qRT-PCR was performed using Student’s t test: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.