FIG 5.

Intestinal microbiome of elders with Alzheimer’s disease induce significantly lower P-glycoprotein expression from intestinal epithelia cells, reflecting a higher level of inflammatory potential at the epithelial cell surface. (A and B) Supernatants were collected from stool samples from 9 randomly selected elders from each dementia classification group and incubated on T84 epithelial cells for 12 h prior to relative quantitation of P-gp by flow cytometry (A) or MRP2 by Western blotting (B). (A) P-glycoprotein expression was induced significantly less in samples from elders with Alzheimer’s disease (AD, yellow) than from those without dementia (none, gray) or with other dementia types (other, blue). Normalized P-glycoprotein (P-gp) expression is expressed on the y axis. (B) MRP2 did not show a significant difference in expression levels. ***, P < 0.05.