FIG 2.

Identification of PP_4108 as an l-2AA aminotransferase. (A) Growth of wild-type KT2440 and the PP_4108 mutant on 2AA as a sole carbon source. The shaded area represents the 95% confidence interval (CI); n = 3. (B) In vivo accumulation of 2AA in wild-type KT2440 and a PP_4108 mutant after 12 h of growth on minimal medium supplemented with 10 mM glucose and 10 mM d-lysine. Bars represent 1og₁₀ transformed spectral counts; error bars show 95% CI; n = 3. (C) In vitro transamination reactions of PP_4108 with 2KG as an amino acceptor. Bars represent metabolite concentrations (in micromoles) of either 2OA (black) or 2AA (white) in either boiled or native protein reactions. Error bars show 95% CI; n = 3. (D) In vitro transaminations of PP_4108 incubated with different possible amino donors and 2KG as the acceptor. Bars represent relative activity levels of enzyme standardized to l-2AA after 16 h of incubation. Error bars show standard deviations; n = 2.