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Assembly of stress fibers in NRK/ras cells requires Rho-kinase activity. NRK/ras cells were treated with 0.1% DMSO or 25 μM U0126 for 48 h. Cells were then further incubated in the absence (−) or presence (+) of 10 μM Y27632 for 1 h and fixed. Actin and vinculin were labeled as described in Figure 1. Bar, 20 μm.
