Figure 8.

Overexpression of a dominant-negative Rho-kinase prevents assembly of stress fibers in NRK/ras cells treated with MEK inhibitors. NRK/ras cells were transiently transfected with pEF-Bos-myc-Rho-kinase-RB/PH(TT). After 24 h, cells were treated with 25 μM U0126 for 30 h, then fixed. Actin was stained as described in Figure 1. Note that nontransfected cells (arrowhead) form stress fibers, whereas Rho-kinase-RB/PH(TT)–transfected cells (arrow) are devoid of stress fibers. Bar, 20 μm.