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. 2019 May 3;10:984. doi: 10.3389/fimmu.2019.00984

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Copyright © 2019 Huang, Zhao, Liu, Xu, Su, Qin and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of rplF- and flgD-RNAi strains and their effects on virulence. (A) Screening of shRNAs targeting rplF and flgD transcripts. Data are presented as mean ± S.D. (n = 3). **P < 0.01, ***p < 0.0001. (B) Expression level of rplF gene of P. plecoglossicida in the spleen of E. coioides. (C) Expression level of rplF gene of P. plecoglossicida in the spleen of E. coioides. (D) The cumulative survival of E. coioides injected with Wild type and RNAi strains during 10 days post challenge. (E) E. coioides that received rplF- or flgD-RNAi strains failed to develop visible nodules.