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. 2019 May 3;10:984. doi: 10.3389/fimmu.2019.00984

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Copyright © 2019 Huang, Zhao, Liu, Xu, Su, Qin and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The virulence of wild-type and htpG-RNAi strain of P. plecoglossicida. (A) Survival rate of E. coioides infected by P. plecoglossicida (n = 3). (B) Symptoms of spleen of E. coioides infected by P. plecoglossicida. (C) Spatial and temporal distribution of htpG-RNAi strain compared to wild-type strain. (D) Expression level of htpG of P. plecoglossicida in the spleen of E. coioides. (E) qRT-PCR analysis of the expression of randomly selected novel genes among wild type P. plecoglossicida in vitro, wild type P. plecoglossicida in vivo, and htpG-RNAi strain in vivo. Data are presented as mean ± S.D. (n = 3). **P < 0.01, ***p < 0.0001.