Figure 2.

Comparison of whole cell patch clamp recordings from both acutely dissected and explant cultured vestibular hair cells. (A) Voltage-activated currents from the crista of a mouse type II vestibular hair cell, prepared as a vestibular triad explant preparation that was cultured for 2 days. (B) Voltage-activated currents from the crista of a mouse type II vestibular hair cell from an acute vestibular triad preparation (2 h post dissection). (C) An IV plot of instantaneous tail current amplitudes (measured at asterisks in A,B) from hair cells in cultured explant preparation (blue trace) and the acute vestibular triad preparation (red trace). The voltage-activated instantaneous tail currents from the explant preparation are comparable to those obtained from an acute preparation and are consistent with recordings from type II vestibular hair cells.