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. 2019 May 3;13:170. doi: 10.3389/fncel.2019.00170

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Copyright © 2019 Ogier, Burt, Drury, Lim and Nayagam.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phalloidin stained cochlear hair cells. (A) One row of inner hair cells (IHC) and three rows of outer hair cells (OHC) illustrating normal polarity and patterning of cochlear stereocilia bundles. Scale bar = 10 μm. (B–F) The length of inner hair cell stereocilia (green) increases from the basal to apical regions of the cochlea. Conversely, stereocilia width decreases from the cochlear base to apex. Images were progressively collected from the basal region of the explant (B) through to the mid-section (D) through to the apex (F) to demonstrate the graded change in stereocilia length and width. Scale bar = 5 μm. Tissues mounted using Prolong Diamond anti-fade reagent and coverslip #1.5.