Table 1.

Recommended reagent list for the dissection and culture of mammalian inner ear hair cells.

Reagent	Supplier	Catalogue number	Recommended storage
MEM	Life Technologies	42360-032	49 ml aliquots at −20°C
DMEM/F12/GlutaMAX	Thermofisher	10565-018	4°C
Non-essential amino acids	Life Technologies	11140-050	500 μl aliquots at 4°C
Pen/Strep	Life Technologies	15140-148	500 μl aliquots at −20°C
Penicillin	Sigma Aldrich	P3032	Aliquots at −20°C
Neurobasal-A medium	Life Technologies	10888-022	49 ml aliquots at −20°C
N2 supplement	Life Technologies	17502-048	500 μl aliquots at −20°C
L-glutamine	Life Technologies	25030-149	500 μl aliquots at −20°C
D-glucose	Life Technologies	15023-021	75 mg aliquots at room temperature
Steriflip disposable vacuum filters	Merck	SE1M179M6	Room temperature
Millicell cell culture insert, 30 mm, hydrophilic PTFE, 0.4 μm	Merck	PICM03050	Room temperature
6 well culture plate	Thermofisher	140675	Room temperature
4% PFA	As preferred		As preferred
Phosphate buffered saline	As preferred		As preferred
Myosin-VII antibody (Rabbit anti-mouse)	Sapphire Bioscience	PTS-25-6790-C050	−20°C
Goat anti-rabbit IgG HandL Alexa Fluor 488	Sapphire Bioscience	AB150085	−20°C
Prolong diamond anti-fade with DAPI	Life Technologies	P36962	Room temperature
Prolong gold anti-fade with DAPI	Life Technologies	P36941	Room temperature
Phalloidin stain	Thermofisher	A12379	−20°C
PTFE printed Microscope slides	Pro Sci Tech	G35068	N/A
Coverslips	Pro Sci Tech	G417	N/A
In situ cell death detection kit	Sigma Aldrich	11684795910	−20°C
Triton X-100	Sigma Aldrich	X100-500ml	Room temperature
Goat Serum	Sapphire Bioscience	AB156046	500 μl aliquots at −20°C
10% neutral buffered formalin	Thermo Fisher Scientific	FNNJJ0185B	Room temperature