Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 9;10:2119. doi: 10.1038/s41467-019-10146-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Heterogeneity of chromatin marks correlates to unresponsiveness to trans-activation. a Levels of H3K9 trimethylation on the Sox1 promoter differ significantly. Sox1^GFP-positive and -negative samples were sorted to perform ChIP for H3K9me3. Gapdh, Oct4, and Actc1 are included as controls. Levels of K9 trimethylation were significantly higher in the Sox1^GFP-negative population compared to the GFP-positive population. Data shown as mean and standard error of the mean of n = 3 biological replicates, performed on different days in different clonal lines, *p < 0.05 in two-sided Student’s t-test. b Levels of H3K27 trimethylation on the Sox1 promoter are similar. Sox1^GFP-positive and -negative samples were sorted to perform ChIP for H3K27me3. Gapdh, Oct4, and Actc1 are included as controls. Levels of K27 trimethylation are not significantly different between the Sox1^GFP-negative and-positive populations. Data shown as the mean and standard error of the mean of n = 3 technical replicates; n.s., not significant in two-sided Student’s t-test. c Sox1^GFP-positive NPCs exhibit lower DNA methylation at the Sox1 promoter. (Top left) Structure of the Sox1 promoter, locations of ChIP qPCR and BS and OxBS amplicons are shown. (Top right) Sequence of the oxBS and BS analyzed sequence in the Sox1 promoter. CpGs are marked in bold. Predicted binding sites of YY1 (marked in grey) and Sp1/Sp3 (marked in pink) as well as E2F-1 (boxed) are indicated. (Below). Sox1^GFP-positive and -negative cells were sorted to perform oxidative bisulfite sequencing. This revealed elevated DNA methylation levels in non-responsive cells on the Sox1 promoter (data depict the mean and standard error of the mean of NGS reads of at least n = 1000 individual sequences generated from two individual biological replicates generated and analyzed on separate days)