Figure 4.

Ipl1 Still Localizes at the Inner Kinetochore with bir1Δ sli15ΔN, and This Is Dependent on COMA

(A) bir1Δ sli15ΔN (sli15ΔN; deletion of 2–228 aa) shows synthetic growth defects when combined with Mcm21 depletion. Yeast cells shown here were serially diluted (10 times each), spotted on plates, and incubated for 2 days in the presence (right) and absence (left) of NAA.

(B) Ipl1 localizes at the inner kinetochore with bir1Δ sli15ΔN dependent on COMA. BIR1⁺SLI15⁺MCM21⁺ (wild-type; T12248), bir1Δ sli15ΔN (T12229), mcm21-aid TIR (T12738), and bir1Δ sli15ΔN mcm21-aid TIR (T12739) cells with IPL1-GFP, GAL1-10 promoter-CEN3-tetOs, TetR-3×CFP, mCherry-TUB1, and MET3 promoter-CDC20 were treated and analyzed as in Figure 2B. Immediately after reactivation of CEN3, images were acquired for 10 min with a 1-min interval. Representative images (left) of T12229 and T12739 cells are shown at top and bottom, respectively. Ipl1 signals were quantified at CEN3 in n = 15–25 cells for each strain (graph at right). Bars show means and SEMs. p values were obtained using t test.

See also Figure S3C.