Fig. 4.

Biolistic-mediated genetic transformation of maize inbred UMI29. a Immature embryos on N6 medium containing 2,4-D (1.0 mg/L) and AgNO₃ (10 mg/L), b immature embryos kept on osmoticum medium containing mannitol (36.4 g/L) and sorbitol (36.4 g/L) 4 h prior to the bombardment, c immature embryo-derived calli after the first round of selection on N6 selection medium (PPT 3 mg/mL), d immature embryo-derived calli after the first round of selection on N6 selection medium (PPT 3 mg/mL), e immature embryo-derived calli after the second round of selection on regeneration medium (PPT 3 mg/mL), f regeneration of shoots on MS medium containing BAP (10 mg/L) and PPT (3 mg/L), g plantlets on half strength MS basal medium containing PPT (3 mg/L), h putative transgenic plants in transgenic greenhouse