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Percentage of cells undergoing apoptosis after DNA replication Quantification was performed by simultaneous staining for DNA synthesis and apoptotic nuclear morphology after UV-C irradiation (36 h post-treatment; 10 J/m2) of 27-1 cells. BrdU-labelling had been performed for 36 h after UV-C irradiation.
| Expt. | Analyzed apoptotic cells | BrdU-positive apoptotic cells | % |
|---|---|---|---|
| 1 | 217 | 165 | 76, 0 |
| 2 | 122 | 77 | 63, 1 |
| 3 | 247 | 173 | 70, 0 |
