Antibodies |
|
Rabbit polyclonal anti-Alexa Fluor-488 |
Thermo Fisher Scientific |
Cat# A-11094; RRID: AB_221544
|
|
Bacterial and Virus Strains |
|
Escherichia coli HB101 strain |
Caenorhabditis Genetics Center (CGC) |
HB101 strain |
E. coli HT115 (DE3) RNAi strain - empty vector |
Julie Ahringer lab |
N/A |
E. coli HT115 (DE3) RNAi strain - unc-22 (ZK617.1) |
Julie Ahringer lab |
N/A |
|
Biological Samples |
|
Commercial raw RJ |
Well Bee-ing UK |
50 gr pure fresh royal jelly: https://www.royaljellyinhoney.co.uk/buy-royal-jelly.html
|
|
Chemicals, Peptides, and Recombinant Proteins |
|
Recombinant full-length MRJP-3 |
This paper |
N/A |
Recombinant truncated MRJP-3 |
This paper |
N/A |
|
Deposited Data |
|
Raw RNA-seq data |
This paper |
ArrayExpress: E-MTAB-6732
|
Raw imaging data |
This paper |
Mendeley Data: https://doi.org/10.17632/5w7rbd8452.1
|
|
Experimental Models: Cell Lines |
|
Sf9 (insect; Spodoptera frugiperda) |
AATC |
ATCC Cat# CRL-1711; RRID: CVCL_0549 |
|
Experimental Models: Organisms/Strains |
|
Caenorhabditis elegans wild type strain |
Caenorhabditis Genetics Center (CGC) |
Wild type strain N2 (var Bristol) |
|
Oligonucleotides |
|
List and sequences of RNA oligos Table S4A) |
This paper |
N/A |
List and sequences of primers (Table S4B) |
This paper |
N/A |
|
Recombinant DNA |
|
Baculovirus expression vector pVL1393 |
Expression Systems |
Cat# 91-013 |
|
Software and Algorithms |
|
RNA-seq analysis scripts |
This paper |
https://doi.org/10.5281/zenodo.1542860 |
GNU R 3.4.4 |
R Development Core Team (2018) |
https://www.r-project.org/ |
STAR 5.2.5b |
Dobin et al., 2013 |
https://github.com/alexdobin/STAR |
samtools |
Li et al., 2009 |
https://github.com/samtools |
cutadapt 1.11 |
Martin, 2011 |
https://cutadapt.readthedocs.io/en/stable/installation.html |
bedtools 2.27.1 |
Quinlan and Hall, 2010 |
https://github.com/arq5x/bedtools2/releases/tag/v2.27.1 |
Subread featureCounts 1.5.0-p2 |
Liao et al., 2013 |
http://subread.sourceforge.net/ |
goseq 1.28 |
Young et al., 2010 |
https://bioconductor.org/packages/release/bioc/html/goseq.html |
|
Other |
|
Labeled ribonucleotide Alexa Fluor 488-5-UTP |
Thermo Fisher Scientific |
Cat# C11403
|
EnvisionTM Flex kit system |
Agilent |
Cat# K802421-2 |
MEGAscript T7 Transcription Kit |
Thermo Fisher Scientific |
Cat# AM1334 |
Ulysis Alexa Fluor 488/647 Nucleic Acid Labeling Kits |
Thermo Fisher Scientific |
Cat# U21650
|
Cat# U21660
|
TruSeq Stranded Total RNA Library Prep kit |
Illumina |
Cat# 20020596 |
ULTRA BEE pollen substitute |
ManLake |
FD-374 |