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. 2019 May 2;74(3):598–608.e6. doi: 10.1016/j.molcel.2019.03.010
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit polyclonal anti-Alexa Fluor-488 Thermo Fisher Scientific Cat# A-11094; RRID: AB_221544

Bacterial and Virus Strains

Escherichia coli HB101 strain Caenorhabditis Genetics Center (CGC) HB101 strain
E. coli HT115 (DE3) RNAi strain - empty vector Julie Ahringer lab N/A
E. coli HT115 (DE3) RNAi strain - unc-22 (ZK617.1) Julie Ahringer lab N/A

Biological Samples

Commercial raw RJ Well Bee-ing UK 50 gr pure fresh royal jelly: https://www.royaljellyinhoney.co.uk/buy-royal-jelly.html

Chemicals, Peptides, and Recombinant Proteins

Recombinant full-length MRJP-3 This paper N/A
Recombinant truncated MRJP-3 This paper N/A

Deposited Data

Raw RNA-seq data This paper ArrayExpress: E-MTAB-6732
Raw imaging data This paper Mendeley Data: https://doi.org/10.17632/5w7rbd8452.1

Experimental Models: Cell Lines

Sf9 (insect; Spodoptera frugiperda) AATC ATCC Cat# CRL-1711; RRID: CVCL_0549

Experimental Models: Organisms/Strains

Caenorhabditis elegans wild type strain Caenorhabditis Genetics Center (CGC) Wild type strain N2 (var Bristol)

Oligonucleotides

List and sequences of RNA oligos Table S4A) This paper N/A
List and sequences of primers (Table S4B) This paper N/A

Recombinant DNA

Baculovirus expression vector pVL1393 Expression Systems Cat# 91-013

Software and Algorithms

RNA-seq analysis scripts This paper https://doi.org/10.5281/zenodo.1542860
GNU R 3.4.4 R Development Core Team (2018) https://www.r-project.org/
STAR 5.2.5b Dobin et al., 2013 https://github.com/alexdobin/STAR
samtools Li et al., 2009 https://github.com/samtools
cutadapt 1.11 Martin, 2011 https://cutadapt.readthedocs.io/en/stable/installation.html
bedtools 2.27.1 Quinlan and Hall, 2010 https://github.com/arq5x/bedtools2/releases/tag/v2.27.1
Subread featureCounts 1.5.0-p2 Liao et al., 2013 http://subread.sourceforge.net/
goseq 1.28 Young et al., 2010 https://bioconductor.org/packages/release/bioc/html/goseq.html

Other

Labeled ribonucleotide Alexa Fluor 488-5-UTP Thermo Fisher Scientific Cat# C11403
EnvisionTM Flex kit system Agilent Cat# K802421-2
MEGAscript T7 Transcription Kit Thermo Fisher Scientific Cat# AM1334
Ulysis Alexa Fluor 488/647 Nucleic Acid Labeling Kits Thermo Fisher Scientific Cat# U21650
Cat# U21660
TruSeq Stranded Total RNA Library Prep kit Illumina Cat# 20020596
ULTRA BEE pollen substitute ManLake FD-374