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. 2019 May 3;10:462. doi: 10.3389/fphar.2019.00462

Figure 6.

Figure 6

Effect of MT1-MMP silencing in MDA-MB-231 on cell growth and survival in 3D type I collagen matrices. (A) Western blot analysis confirming efficient knock down of MT1-MMP in MDA-MB-231 shMT1-MMP. GAPDH was used as a loading control. (B–E) MDA-MB-231 shCtrl and MDA-MB-231 shMT1-MMP cells were embedded in 3D type I collagen matrices. After 36 h of culture, cell extracts were analyzed for tyrosine phosphorylation of DDR1 and total DDR1 by Western blotting. GAPDH was used as a loading control (B). After 5 days of culture, viable cell density was evaluated by phase contrast microscopy (C). After 36 h of culture, apoptosis was quantified using the Muse® annexin V and dead cell assay kit (D) and BIK expression was measured by RT-PCR (E). Values represent the mean ± S.D. of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001).