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. 2019 May 3;10:462. doi: 10.3389/fphar.2019.00462

Figure 7.

Figure 7

Effect of DDR1 expression in MDA-MB-231 on cell growth and survival in 3D type I collagen matrices. (A) Western blot analysis of ectopically expressed DDR1-GFP in MDA-MB-231. *: full-length endogenous DDR1. GAPDH was used as a loading control. (B) MDA-MB-231 VEC GFP (control GFP) and MDA-MB-231 DDR1-GFP cells were embedded in type I collagen 3D matrices. After 5 days of culture, viable cell density was evaluated by phase contrast microscopy. (C) MDA-MB-231 VEC GFP (control GFP) and MDA-MB-231 DDR1-GFP cells were embedded in 3D type I collagen matrices in absence (Ctrl) or in presence of nilotinib (100 nM). After 36 h of culture, BIK expression was measured by RT-PCR. Values represent the mean ± S.D. of three independent experiments (***p < 0.001).