Figure 1.

A, HEK-293T cells were transfected with Myc-Tat–expressing plasmid; 24 h post-transfection, the cells were either treated with CHX (100 μg/ml) alone or CHX with ammonium chloride or MG132 for the indicated time periods. Tat protein level was measured by immunoblotting. The blot shown is representative of three different independent experiments. B, Tat protein intensity was measured using ImageJ and plotted with reference to CHX treatment time.